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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Dev Cell. 2013 Feb 25;24(4):411–425. doi: 10.1016/j.devcel.2013.01.018

Figure 4. Rpl22l1 knockdown blocks HSC emergence and definitive hematopoiesis.

Figure 4

(A,B) The effect of Rpl22l1 knockdown on development of definitive erythroid (A; a-e1globin) and myeloid (B; lyc) progenitors was assessed by WISH with the indicated probes at 5dpf in embryos injected with Rpl22l1 MO or MM control. Developing kidney, boxed area and asterisk; CHT, blue arrowheads; VDA, red arrowheads.

(C–E) Effect of Rpl22l1 knockdown on c-myb and Runx expressing progenitors. Embryos were injected with MO as above and evaluated by WISH at 5dpf (C), 36hpf (D), and 24hpf (E) with the indicated probes. Developing kidney (C; red boxed insert, blue asterisk); thymus (C; red boxed inset, red circle); AGM, (D; arrows); AGM (E; blue boxed area, arrows); and erythro-myeloid progenitors (E; EMP, red boxed inset, red arrowhead).

(F) Impact of Rpl22l1 knockdown on the vasculature. Dorsal aorta (efnb2a), vasculature (flk1) and pronephros (cdh17) were assessed by WISH in control and rpl22l1 morphant embryos as above with the indicated probes (arrows).

Images depict phenotypes representative of at least 3 separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure S4.