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. Author manuscript; available in PMC: 2014 Feb 25.
Published in final edited form as: Dev Cell. 2013 Feb 25;24(4):411–425. doi: 10.1016/j.devcel.2013.01.018

Figure 7. Opposing roles of Rpl22 and Rpl22l1 in regulating Smad1 expression and HSC emergence.

Figure 7

(A,B) Effect of Rpl22 and Rpl22l1 overexpression on Smad1 protein levels and HSC emergence. 100pg of RNA encoding rpl22, rpl22l1, or both were injected into embryos and the effect on Smad1 protein levels assessed by western blotting at 24 hpf (A). β-Actin served as a loading control. The effect on HSC emergence in the AGM was assessed by WISH for runx1 at 24 hpf (B).

(C,D) Requirement of Rpl22 RNA binding ability in regulating Smad1 expression and HSC emergence. 75ng of mRNA encoding intact or RNA binding mutant rpl22 (m88) was injected into embryos and the effect on Smad1 protein levels was assessed by blotting at 24hpf, as above. The effect on smad1 mRNA levels was determined using real time PCR and the mean ± S.D was depicted graphically (C). The effect on HSC emergence was assessed by WISH as above (D).

(E–G) Rescue of developmental arrest in rpl22l1 morphants by knockdown of Rpl22. 1ng of MO targeting Rpl22, Rpl22l1, or the two together (D-MO) were injected into zebrafish embryos, following which Smad1 protein expression was assessed at 24hpf as above (E). The effect on HSC emergence was assessed by WISH at 24hpf (runx1) and 36 hpf (c-myb) and the effect on thymus seeding was assessed at 5dpf using the Tg(ikaros:EGFP) to mark thymic progenitors. VDA (F, red arrowheads); Thymus (G, red circle)

(H) Model of Rpl22 and Rpl22l1 function in hematopoiesis. Our analysis indicates that Rpl22l1 promotes runx1 induction and HSC emergence by facilitating Smad1 expression and BMP signaling. The promotion of Smad1 expression by Rpl22l1 is antagonized by Rpl22, perhaps through direct binding of Smad1 mRNA. In contrast, Rpl22 is dispensable for the emergence of HSC and consequent seeding of the thymus by progenitors; however, Rpl22 plays a critical role in supporting the development of thymic progenitors after seeding, by suppressing p53 expression in a lineage-restricted manner.

Images depict phenotypes representative of at least 3 separate experiments, with numbers referring to the fraction of morphants with the depicted phenotypes. See also Figure S6.