Table I.
MicroRNA differentially expressed in whole mouse blood after exposure to ionizing radiation in vivo. Mice were irradiated with γ-rays (doses of 0, 0.5, 1.5, or 5.0 Gy) or 56Fe particles (doses of 0, 0.1, or 0.5 Gy), 250 µl blood was collected directly in lysis solution, and total RNA was purified 6 and 24 h after irradiation. miRNA expression levels were determined by 384-well low-density TaqMan® real-time PCR miRNA expression assays. The expression data was preprocessed and normalised. Fold changes in expression level, relative to non-irradiated control samples, with a false discovery rate of < 0.07 were considered to be statistically significant.
| Irradiation condition | miRNA ID | Only expressed in one condition?a |
Fold change ± SEM |
p-value | FDR |
|---|---|---|---|---|---|
| γ 6 h 0.5 Gy | miR-511 | R | 197.0 ± 1.18 | 5.2E-08 | 9.4E-06 |
| miR-598 | R | 20.0 ± 2.89 | 1.6E-06 | 1.4E-04 | |
| γ 6 h 1.5 Gy | miR-135a | 4.0 ± 1.26 | 6.3E-04 | 0.0182 | |
| miR-147 | R | 80.6 ± 3.30 | 3.1E-07 | 2.7E-05 | |
| miR-150 | −2.2 ± 1.17 | 0.0028 | 0.0676 | ||
| miR-200b | C | −686.4 ± 28.72 | 2.1E-07 | 2.7E-05 | |
| miR-547 | C | −18.8 ± 4.14 | 9.9E-06 | 5.6E-04 | |
| miR-680 | R | 182.8 ± 1.59 | 1.3E-05 | 5.6E-04 | |
| miR-685 | R | 170.0 ± 12.34 | 3.6E-05 | 0.0029 | |
| miR-708 | C | −43.0 ± 5.35 | 2.2E-04 | 0.0075 | |
| γ 6 h 5.0 Gy | miR-10a | 1.8 ± 1.08 | 0.0027 | 0.0529 | |
| miR-135a | 19.9 ± 1.15 | 8.0E-07 | 4.6E-05 | ||
| miR-135b | 8.1 ± 1.54 | 0.0010 | 0.0267 | ||
| miR-139-5p | 3.8 ± 1.23 | 4.0E-04 | 0.0140 | ||
| miR-147 | R | 215.0 ± 3.24 | 0.0011 | 0.0267 | |
| miR-223 | 3.6 ± 1.09 | 4.1E-05 | 0.0018 | ||
| miR-450a-5p | 2.2 ± 1.12 | 0.0012 | 0.0267 | ||
| miR-680 | R | 2530.0 ± 1.35 | 4.9E-08 | 4.3E-06 | |
| miR-685 | R | 2430.0 ± 12.55 | 5.9E-09 | 1.0E-06 | |
| γ 24 h 1.5 Gyb | miR-335-3p | 4.2 ± 1.25 | 2.6E-04 | 0.0229 | |
| miR-667 | C | −91.8 ± 6.05 | 4.5E-09 | 8.0E-07 | |
| γ 24 h 5.0 Gy | miR-125a-3p | 3.6 ± 1.35 | 0.0020 | 0.0327 | |
| miR-146a | −3.9 ± 1.33 | 0.0011 | 0.0221 | ||
| miR-150 | −19.7 ± 1.39 | 1.5E-05 | 5.3E-04 | ||
| miR-151-3p | 2.5 ± 1.21 | 0.0031 | 0.0453 | ||
| miR-211 | C | −377.2 ± 5.97 | 3.0E-07 | 1.3E-05 | |
| miR-335-3p | 3.7 ± 1.27 | 7.5E-04 | 0.0166 | ||
| miR-337-3p | C | −30.0 ± 3.67 | 1.4E-04 | 0.0042 | |
| miR-339-3p | 3.1 ± 1.24 | 0.0013 | 0.0235 | ||
| miR-342-3p | −6.5 ± 1.39 | 3.3E-04 | 0.0083 | ||
| miR-501-3p | R | 12300.0 ± 1.18 | 3.3E-10 | 5.9E-08 | |
| miR-511 | R | 107.0 ± 6.45 | 1.1E-07 | 9.6E-06 | |
| miR-667 | C | −100.1 ± 4.22 | 2.4E-07 | 1.3E-05 | |
| 56Fe 6 h 0.1 Gy | miR-125a-3p | C | −117.1 ± 3.75 | 1.5E-08 | 2.7E-06 |
| miR-350 | −1.7 ± 1.06 | 3.7E-04 | 0.0169 | ||
| miR-379 | R | 680.3 ± 1.61 | 8.1E-06 | 5.0E-04 | |
| miR-383 | C | −21.5 ± 1.24 | 6.9E-06 | 5.0E-04 | |
| 56Fe 6 h 0.5 Gy | miR-383 | C | −20.2 ± 1.25 | 4.4E-06 | 2.7E-04 |
| miR-501-3p | R | 336.9 ± 16.75 | 1.7E-10 | 3.1E-08 | |
| miR-680 | R | 107.6 ± 7.62 | 5.2E-07 | 4.7E-05 | |
| 56Fe 24 h 0.1 Gy | miR-409-3p | R | 64.1 ± 6.47 | 1.0E-08 | 9.8E-07 |
| miR-685 | R | 292.8 ± 10.76 | 7.0E-10 | 1.3E-07 | |
| 56Fe 24 h 0.5 Gy | miR-150 | −2.5 ± 1.17 | 8.6E-04 | 0.0326 | |
| miR-211 | −2.2 ± 1.10 | 2.5E-04 | 0.0232 | ||
| miR-342-3p | −2.2 ± 1.12 | 4.4E-04 | 0.0278 | ||
| miR-494 | −2.4 ± 1.17 | 8.1E-04 | 0.0326 | ||
| miR-879 | R | 35.3 ± 6.15 | 1.8E-08 | 3.4E-06 |
ID, identification; SEM, standard error of the mean; FDR, false discovery rate.
MicroRNA that are only expressed in the sham-irradiated controls (C) or after exposure to ionising radiation (R).
No miRNA were differentially expressed after 0.5 Gy γ irradiation at the 24 h time point.