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Immunofluorescence staining of human brain microvascular endothelial cells [HBMECs] showing progressive increase in the number of Neospora caninum tachyzoites over time. Cell nuclei [blue] and N. caninum tachyzoites [green]. Staining was performed as described in the methods at various time points post infection as indicated in the figure. (Scale bar = 20 μl.).
