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False positive and recovery performance. a) On average 1.2 cells were found per mL of healthy donor blood with 60% of the samples reporting zero cells. b) MCF-7 cells with known numbers were spiked into a healthy donor’s blood, which was then labeled with PE-anti-EpCAM, Alexa647-anti-cytokeratin, and FITC-anti-CD45. Enumeration results showed an average 94% recovery.
