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(a) Coexpression of CFP-NaPi2a and GFP-PIST in OK cells. The two proteins colocalize intracellularly at the site of PIST expression. (b) Expression of the endogenous NaPi-2a in OK cells (NaPi-4) is only clearly seen in BBM under control conditions. (c) Overexpression of PIST restricts the expression of NaPi4 to the TGN. (d) Pi transport in OK cells transfected with GFP-PIST is reduced proportionally to the efficiency of PIST transfection. Dashed line: level of Na-independent uptake.
