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Changes in enthalpy (ΔH), entropy (ΔS), Gibbs free energy (ΔG) at 25°C, and denaturation temperature (T m) for rPA unfolding (N↔I) with and without 0.25 M urea.
| ΔH/kJ mol−1 | ΔS/kJ mol−1 K−1 | ΔG at 25°C/kJ mol−1 | T m (ΔG = 0)/°C | |
|---|---|---|---|---|
| rPA without additive | 975.8 ± 40.5 | 3.02 ± 0.13 | 76.1 | 50.2 |
| rPA with 0.25 M urea | 949.7 ± 94.6 | 2.92 ± 0.30 | 77.7 | 51.6 |
