Table 1.
Primary antibody sources, dilution, and antigen retrieval buffers and conditions.
| Antibody | Source | Dilution | Antigen retrieval buffer | Microwaving time |
|---|---|---|---|---|
| Beta-catenin | LEICA NCL-B-CAT | 1 : 100 | Citrate | 30 min |
| p53 | LEICA NCL-p53-D07 | 1 : 100 | Citrate | 20 min |
| Bcl-2 | DAKO M0887 |
1 : 200 | EDTA | 30 min |
| Cyclin-D1 | Thermo Shandon RM-9104-S | 1 : 25 | Citrate | 40 min |
| Caspase-3 | Cell Signalling CAT: 9664 | 1 : 800 | EDTA | 20 min |
| CD10 | LEICA NCL-L-CD10-270 | 1 : 20 | EDTA | 30 min |
| Ki-67 | LEICA NCL-L-KI67-MM1 | 1 : 100 | Citrate | 30 min |