Table 1.
O-linked oligosaccharides identified from a 1-μg aliquot of bile salt- stimulated lipase (BSSL). The structures in red were unique carbohydrates identified via the enzymatic/chemical release procedure.
| Obs. Mass | Calc. Mass | ΔMass | Composition |
|---|---|---|---|
| 866.444 | 866.437 | 0.007 | Hex1HexNAc1Deoxyhexose2 |
| 879.415 | 879.432 | 0.017 | Hex1HexNAc1NeuAc1 |
| 896.446 | 896.447 | 0.001 | Hex2HexNAc1Deoxyhexose1 |
| 937.474 | 937.474 | 0 | Hex1HexNAc2Deoxyhexose1 |
| 967.487 | 967.484 | 0.003 | Hex2HexNAc2 |
| 1083.522 | 1083.532 | 0.01 | Hex2HexNAc1NeuAc1 |
| 1124.557 | 1124.558 | 0.001 | Hex1HexNAc2NeuAc1 |
| 1130.57 | 1130.558 | 0.012 | Hex4HexNAc2 |
| 1141.555 | 1141.573 | 0.018 | Hex2HexNAc2Deoxyhexose1 |
| 1171.572 | 1171.584 | 0.012 | Hex3HexNAc2 |
| 1212.616 | 1212.611 | 0.005 | Hex2HexNAc3 |
| 1240.581 | 1240.606 | 0.025 | Hex1HexNAc1NeuAc2 |
| 1298.649 | 1298.647 | 0.002 | Hex1HexNAc2Deoxyhexose1NeuAc1 |
| 1315.65 | 1315.663 | 0.013 | Hex2HexNAc2Deoxyhexose2 |
| 1328.654 | 1328.658 | 0.004 | Hex2HexNAc2NeuAc1 |
| 1345.648 | 1345.673 | 0.025 | Hex3HexNAc2Deoxyhexose1 |
| 1386.685 | 1386.7 | 0.015 | Hex2HexNAc3Deoxyhexose1 |
| 1416.708 | 1416.71 | 0.002 | Hex3HexNAc3 |
| 1489.706 | 1489.752 | 0.046 | Hex2HexNAc2Deoxyhexose3 |
| 1502.74 | 1502.747 | 0.008 | Hex2HexNAc2Deoxyhexose1NeuAc1 |
| 1519.819 | 1519.762 | 0.057 | Hex3HexNAc2Deoxyhexose2 |
| 1590.788 | 1590.8 | 0.012 | Hex3HexNAc3Deoxyhexose1 |
| 1620.819 | 1620.81 | 0.009 | Hex4HexNAc3 |
| 1661.817 | 1661.837 | 0.02 | Hex3HexNAc4 |
| 1676.812 | 1676.836 | 0.024 | Hex2HexNAc2Deoxyhexose2NeuAc1 |
| 1689.819 | 1689.832 | 0.013 | Hex2HexNAc2NeuAc2 |
| 1747.86 | 1747.873 | 0.013 | Hex2HexNAc3Deoxyhexose1NeuAc1 |
| 1764.873 | 1764.889 | 0.016 | Hex3HexNAc3Deoxyhexose2 |
| 1777.852 | 1777.884 | 0.032 | Hex3HexNAc3NeuAc1 |
| 1794.897 | 1794.899 | 0.002 | Hex4HexNAc3Deoxyhexose1 |
| 1818.85 | 1818.911 | 0.061 | Hex2HexNAc4NeuAc1 |
| 1835.901 | 1835.926 | 0.025 | Hex3HexNAc4Deoxyhexose1 |
| 1865.922 | 1865.936 | 0.014 | Hex4HexNAc4 |
| 1921.966 | 1921.963 | 0.003 | Hex2HexNAc3Deoxyhexose2NeuAc1 |
| 1938.968 | 1938.978 | 0.01 | Hex3HexNAc3Deoxyhexose3 |
| 1951.951 | 1951.973 | 0.022 | Hex3HexNAc3Deoxyhexose1NeuAc1 |
| 1981.942 | 1981.984 | 0.042 | Hex4HexNAc3NeuAc1 |
| 2009.975 | 2010.015 | 0.04 | Hex3HexNAc4Deoxyhexose2 |
| 2039.989 | 2040.026 | 0.037 | Hex4HexNAc4Deoxyhexose1 |
| 2069.996 | 2070.036 | 0.04 | Hex5HexNAc4 |
| 2098.04 | 2098.031 | 0.009 | Hex4HexNAc2NeuAc2 |
| 2113.007 | 2113.067 | 0.06 | Hex3HexNAc3Deoxyhexose4 |
| 2126.046 | 2126.062 | 0.016 | Hex3HexNAc3Deoxyhexose2NeuAc1 |
| 2156.022 | 2156.073 | 0.051 | Hex4HexNAc3Deoxyhexose1NeuAc1 |
| 2214.094 | 2214.115 | 0.021 | Hex4HexNAc4Deoxyhexose2 |
| 2227.082 | 2227.11 | 0.028 | Hex4HexNAc4NeuAc1 |
| 2268.065 | 2268.137 | 0.072 | Hex3HexNAc5NeuAc1 |
| 2300.117 | 2300.152 | 0.035 | Hex3HexNAc3Deoxyhexose3NeuAc1 |
| 2315.135 | 2315.163 | 0.028 | Hex5HexNAc5 |
| 2388.139 | 2388.204 | 0.065 | Hex4HexNAc4Deoxyhexose3 |
| 2401.167 | 2401.199 | 0.032 | Hex4HexNAc4Deoxyhexose1NeuAc1 |
| 2472.168 | 2472.236 | 0.068 | Hex4HexNAc5NeuAc1 |
| 2489.18 | 2489.252 | 0.072 | Hex5HexNAc5Deoxyhexose1 |
| 2562.169 | 2562.047 | 0.122 | Hex4HexNAc4Deoxyhexose4 |
| 2575.224 | 2575.289 | 0.065 | Hex4HexNAc4Deoxyhexose2NeuAc1 |
| 2588.056 | 2588.284 | 0.228 | Hex4HexNAc4NeuAc2 |
| 2663.267 | 2663.341 | 0.074 | Hex5HexNAc5Deoxyhexose2 |
| 2676.226 | 2676.336 | 0.11 | Hex5HexNAc5NeuAc1 |
| 2749.292 | 2749.378 | 0.086 | Hex4HexNAc4Deoxyhexose3NeuAc1 |
| 2762.295 | 2762.373 | 0.078 | Hex4HexNAc4Deoxyhexose1NeuAc2 |
| 2837.277 | 2837.43 | 0.153 | Hex5HexNAc5Deoxyhexose3 |
| 2850.324 | 2850.425 | 0.101 | Hex5HexNAc5Deoxyhexose1NeuAc1 |
| 2938.385 | 2938.478 | 0.093 | Hex6HexNAc6Deoxyhexose1 |
| 3011.371 | 3011.519 | 0.148 | Hex5HexNAc5Deoxyhexose4 |
| 3024.426 | 3024.515 | 0.089 | Hex5HexNAc5Deoxyhexose2NeuAc1 |
| 3112.365 | 3112.567 | 0.201 | Hex6HexNAc6Deoxyhexose2 |
| 3125.429 | 3125.562 | 0.133 | Hex6HexNAc6NeuAc1 |
| 3198.425 | 3198.604 | 0.179 | Hex5HexNAc5Deoxyhexose3NeuAc1 |
| 3211.492 | 3211.653 | 0.161 | Hex5HexNAc5Deoxyhexose1NeuAc2 |
| 3286.418 | 3286.655 | 0.237 | Hex6HexNAc6Deoxyhexose3 |
| 3299.491 | 3299.652 | 0.161 | Hex6HexNAc6Deoxyhexose1NeuAc1 |
| 3372.505 | 3372.691 | 0.186 | Hex7HexNAc7Deoxyhexose1 |
| 3388.388 | 3387.704 | 0.316 | Hex7HexNAc7Deoxyhexose1 |
| 3473.497 | 3473.741 | 0.244 | Hex6HexNAc6Deoxyhexose2NeuAc1 |
| 3647.482 | 3647.83 | 0.348 | Hex6HexNAc6Deoxyhexose3NeuAc1 |