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Micrographs of transfected H9c2(2-1) cells with time. Cells were transfected using naked ODN (A–C), PEI-ODN polyplexes at 5 N/P (D–F) or Glycofect (G4)-ODN polyplexes at 20 N/P (G–I) and fixed at the indicated time. FITC ODNs (green), LysoTracker (red), Draq5 (blue). The confocal pinhole was opened to capture a 1.5 µm vertical slice in order to observe the diffuse cytoplasmic location of ODNs. Scale bar = 20 µm.
