(a) Conventional microcontact printing (top row), and stamp-off (bottom row), for 20 μm features that are spaced 20, 110, or 200 μm apart (edge-to-edge). (b)–(e) A flat stamp was loaded with BSA conjugated to AlexaFluor-594 (red), stamped-off, reloaded with BSA conjugated to AlexaFluor-488 (green), stamped-off, and imaged. (f) A flat stamp was loaded with BSA conjugated to AlexaFluor-594 (red), stamped-off, reloaded with BSA conjugated to AlexaFluor-488 (green), stamped-off, reloaded with BSA conjugated to AlexaFluor-647 (blue), stamped-off, and imaged. All scale bars, 20 μm.