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. Author manuscript; available in PMC: 2013 Mar 3.
Published in final edited form as: Integr Biol (Camb). 2011 Feb 4;3(5):560–567. doi: 10.1039/c0ib00129e

Fig. 2. Advantages of stamp-off.

Fig. 2

(a) Conventional microcontact printing (top row), and stamp-off (bottom row), for 20 μm features that are spaced 20, 110, or 200 μm apart (edge-to-edge). (b)–(e) A flat stamp was loaded with BSA conjugated to AlexaFluor-594 (red), stamped-off, reloaded with BSA conjugated to AlexaFluor-488 (green), stamped-off, and imaged. (f) A flat stamp was loaded with BSA conjugated to AlexaFluor-594 (red), stamped-off, reloaded with BSA conjugated to AlexaFluor-488 (green), stamped-off, reloaded with BSA conjugated to AlexaFluor-647 (blue), stamped-off, and imaged. All scale bars, 20 μm.