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. Author manuscript; available in PMC: 2013 Mar 3.
Published in final edited form as: Integr Biol (Camb). 2011 Feb 4;3(5):560–567. doi: 10.1039/c0ib00129e

Fig. 3. Integrin segregation on multicolor surface patterns.

Fig. 3

(a)–(c) Fluorescent micrographs of BSA conjugated to AlexaFluor-488 (green) to represent vitronectin, BSA conjugated to AlexaFluor-594 (red) to represent collagen type I and black to represent non-adhesive F127. (d)–(f) Micrographs of human umbilical vein endothelial cells (HUVECs), seeded on substrates patterned as in (a)–(c), fixed after 1 h, and immunolabeled. Note the colocalization of αvβ5 integrin to vitronectin, and β1 integrin to collagen type I. All scale bars, 20 μm.