Fig 3.

Induction of S149E-G3BP stress granule assembly by the MK-STYX active mutant. Representative examples of the subcellular distribution of GFP-tagged S149E-G3BP without heat shock (A) or after heat shock at 41 °C (B), in the presence of pMT2 (empty vector), MK-STYX or the active mutant as indicated. Cells were fixed, stained with DAPI and analyzed 24 h post-transfection for heat shock-induced stress granule assembly by fluorescence microscopy. Merged images show the localization of S149E-G3BP (green) relative to the DAPI-stained nucleus (blue). Scale bar = 10 μm. (C) Cells were scored for the presence or absence of stress granules. Three replicate experiments were performed (n = 100 cells for each experiment); error bars indicate the SEM.