Figure 2. Sulforhodamine-B transport across Caco-2 monolayers.

(a) Time-course study of sulforhodamine-B transport (mg) at different loading concentrations. Sulforhodamine-B was loaded in apical chambers at 0.05 (open circles), 0.15 (filled circles), 0.3 (squares), and 0.6 (triangles) mg/well respectively; and apical-to-basolateral permeation was measured by measuring the fluorescence in samples collected from basolateral chamber at different time-points up to 5 hrs. (b) % Sulforhodamine-B transport across Caco-2 monolayers over of 5 hrs of incubation. Data represent mean±SD (n = 3).