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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: Int J Dev Biol. 2012;56(5):325–332. doi: 10.1387/ijdb.120030jw

Fig. 4. Mutational analysis of the ecmA 39-mer.

Fig. 4

The CA-rich sequences (CA1-3; underlined) within the 39-mer region of ecmA376:lacZ were mutated, by C to A substitutions as indicated by small case letters, to generate CA1m:lacZ-CA3m:lacZ (Fig. 1A and Fig. 3). The staining patterns of parental ecmA376:lacZ and each of the three mutant constructs are shown. They were all stained for 16 hours. CA1m:lacZ and CA3m:lacZ showed almost no staining by 8 hours of incubation, at which time the wild-type control (ecmA376:lacZ) showed strong labeling in pstA cells (data not shown). Bar represents 200 μm.