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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: Development. 2008 Aug 13;135(18):3093–3101. doi: 10.1242/dev.026377

Fig. 7. Yeast two-hybrid analysis of CudA homodimerisation.

Fig. 7

The CudA prey is constitutively expressed and in the presence of galactose as an inducer (in Gal+ medium) the bait is also expressed. The test bait is CudA and the STATa SH2 domain is used as a negative control bait. Because there is an interaction between bait and prey, i.e. homodimerisation of CudA, lacZ expression is activated and is detected with X-Gal. Staining was for 3 days at 30°C. The same bait and prey combinations were tested by growth selection using a leu marker and gave identical results (data not shown).