Table 1.
Clinical and Laboratory Characteristics of 168 Subjects
| Characteristics | Absolute value | % |
|---|---|---|
| Age at sample (years)∗ | 58 (27-83) | |
| Ethnic origin | ||
| White American | 153 | 91.1 |
| African American | 8 | 4.8 |
| Hispanic | 6 | 3.6 |
| Asian | 1 | 0.6 |
| Sex | ||
| Male | 110 | 65.5 |
| Female | 58 | 34.5 |
| Rai stage | ||
| ≤2 | 132 | 78.6 |
| ≥3 | 36 | 21.4 |
| WBC at sample date (109/L)∗ | 76.2 (7.9-372) | |
| IGHV somatic mutation status | ||
| Mutated | 90 | 53.6 |
| Unmutated | 76 | 45.2 |
| Not available | 2 | 1.2 |
| B2M (mg/L) | ||
| ≤4 | 122 | 72.6 |
| >4 | 46 | 27.4 |
| Median (range) | 3.3 (1.3-7.8) | |
| ZAP70 expression† | ||
| Positive | 73 | 43.5 |
| Negative | 73 | 43.5 |
| Not available | 22 | 13.1 |
| CD38 expression (%)‡ | ||
| <30 | 117 | 69.6 |
| ≥30 | 45 | 26.8 |
| Not available | 6 | 3.6 |
| Light chain use | ||
| κ | 104 | 61.9 |
| λ | 58 | 34.5 |
| Not available | 6 | 3.6 |
| FISH positivity (hierarchical model) | ||
| Del13q as sole abnormality | 35 | 20.8 |
| Trisomy 12 | 21 | 12.5 |
| Del11q | 17 | 10.1 |
| Del17p | 6 | 3.6 |
| Normal | 32 | 19.1 |
| Not available | 57 | 33.9 |
| Follow-up (months)∗ | ||
| Diagnosis to sample (N = 168) | 30.1 (0.0-211.5) | |
| Diagnosis to first treatment (N = 144)§ | 34.0 (0.7-211.5) | |
| Diagnosis to final follow-up (N = 168)¶ | 138.0 (0.7-271.3) | |
| Sample to final follow-up (N = 168)¶ | 72.6 (0.7-271.3) | |
FISH, fluorescence in situ hybridization; WBC, white blood cell.
Data are given as median (range).
The expression of ZAP70 was analyzed by either immunohistochemistry (96 patients) or flow cytometry (50 patients). By flow cytometry, patients were designated ZAP70 positive if expression was ≥20% in CD19-positive cells.
Immunophenotypic analysis of CD38 expression was assessed on bone marrow for 145 patients and on peripheral blood for 17 patients for whom bone marrow was unavailable.
The time from diagnosis to first treatment was assessed only for the cohort of 144 patients who have received treatment as of the last follow-up. The median was computed using Kaplan-Meier estimates.
Follow-up times are estimated using the reverse Kaplan-Meier method.