Skip to main content
. 2013 Mar;182(3):992–1004. doi: 10.1016/j.ajpath.2012.11.008

Figure 1.

Figure 1

Cav1 overexpression decreases in vitro proliferation and in vivo tumor incidence and growth in PAM212 keratinocytes. A: Western blot analysis of PAM212 cells retrovirally transduced with either empty pBabe plasmid (pBabe) or pBabe-Cav1 overexpression plasmid (Cav1) shows increased Cav1 expression, but no change in the expression of Cav2; K14, a marker of proliferating keratinocytes; or K10, a marker of differentiating keratinocytes. B: Cav1 PAM212 cells display reduced proliferation, as determined by a BrdU incorporation assay (4 hours; n = 16 per group) and decreased expression of cyclin D1 and p-histone H3 proteins. Cav1 overexpression dramatically reduces tumor incidence (C) and growth (D) in PAM212 cells intradermally injected in the back skin of mice (n = 10 per group). †P < 0.05 (by Fisher’s exact test). Inset: Tumor growth 4 weeks after injection. Results are reported as means ± SEM. *P < 0.05, **P < 0.01 by the unpaired t-test. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.