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. 2013 Mar;182(3):992–1004. doi: 10.1016/j.ajpath.2012.11.008

Figure 2.

Figure 2

Cav1 knockdown increases in vitro proliferation and in vivo tumor growth in PAM212 cells. A: Western blot analysis of PAM212 cells stably transduced via retroviral infection with either scramble shCtl or shCav1. Cav1 knockdown results in a decrease in Cav2 expression, whereas K14 and K10 expression is unaffected. B: Confocal microscopy of shCtl and shCav1 PAM212 cells immunostained with antibodies against Cav1 and Cav2. Hoechst 33342 is shown as a nuclear marker. In the absence of Cav1, Cav2 remains sequestered around the nucleus. C: Cav1 knockdown increases the proliferative ability of PAM212 cells, as determined by a BrdU incorporation assay (4 hours; n = 4 per group) and increased expression of cyclins A and D1. D: In vivo tumor growth was assessed as in Figure 1D. Cav1 knockdown results in a dramatic increase in tumor volume and weight. Inset: Tumor growth 4 weeks after injection. Results are reported as means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001 by the unpaired t-test. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.