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. 2013 Mar;182(3):992–1004. doi: 10.1016/j.ajpath.2012.11.008

Figure 7.

Figure 7

Cav1 expression is decreased in the human cSCC cell line, SCC13, and Cav1 overexpression decreases in vitro proliferation, migration, and invasion. A: Western blot analysis shows that Cav1 expression is decreased in SCC13 compared with the immortalized human keratinocyte cell line, HaCaT. Cav2 expression is also decreased, whereas K14 expression is unaffected. B: Western blot analysis shows successful overexpression of Cav1 in SCC13, whereas Cav2 and K14 expression levels are unaffected. C: Cav1 overexpression decreases cell growth in SCC13, as indicated by a growth curve (n = 3 per group). D: Transwell assays show that overexpression of Cav1 decreases both the migratory and invasive ability of these cells (n = 4 per group). E: Western blot analysis shows that Cav1 overexpression does not affect Erk activation or the expression of K18. Results are reported as means ± SEM. *P < 0.05, **P < 0.01 by the unpaired t-test. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.