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. 2013 Mar;182(3):1031–1042. doi: 10.1016/j.ajpath.2012.11.037

Figure 3.

Figure 3

Shp-2 recruitment is dependent on Src kinase. A: Shp-2 recruitment to coated glass beads was visualized, as in Figure 2, in the presence of 5 μmol/L Src kinase inhibitor, PP2, or 5 μmol/L inactive inhibitor analogue, PP3. Beads were coated with the indicated proteins in addition to a small amount of fluorescently labeled non-specific antibodies to aid in their visualization using immunofluorescence (IF). Arrows, beads with Shp-2 recruitment. Data are representative of three independent experiments. Scale bars: 10 μm. B: The number of beads with enrichment was determined, as in Figure 2B. Data shown are the mean and SD from three independent experiments. *P < 0.01. ns-mIgG, non-specific mouse IgG.