Figure 2. Computer-simulated images with predefined values of colocalization demonstrating its gradual increase (from 0 to 0.9 according to the 0 to 1.0 scale) as indicated by respective scatter grams at the upper right corner showing pixels concentrating along their diagonals as the degree of colocalization rises (a–j).

Images were generated by merging pairs of single-channel red and single-channel green computer-simulated images for the respective pair of channels. Then, they were used to adjust the widths of Gaussian membership functions (see Tables 1 and 2). Images were created using BioSim simulation software (see Methods for details).