Table 1. List of primers developed and used in this study.
Gene | Primer set | Expected size (bp) | Primer sequence (5′to 3′) | Reference |
---|---|---|---|---|
Portiera 16S rDNA | Port-F | 229 | TAGTCCACGCTGTAAACG | 56 |
Port-R | AGGCACCCTTCCATCT | |||
Hamiltonella 16S rDNA | Ham-F | 243 | GCATCGAGTGAGCACAGTT | 57 |
Ham-R | TATCCTCTCAGACCCGCTAA | |||
TYLCV | C473 | 410 | AGTCACGGGCCCTTACA | 48 |
V61 | ATACTTGGACACCTAATGGC | |||
TYLCV (q-PCR) | TY-F | 144 | GTCTACACGCTTACGCC | 16 |
TY-R | GCAATCTTCGTCACCC | |||
β-actin | β-actin F | 130 | TCTTCCAGCCATCCTTCTTG | 40 |
β-actin R | CGGTGATTTCCTTCTGCATT | |||
25S Tomato | 25S rRNA F | 113 | ATAACCGCATCAGGTCTCCA | 39 |
25S rRNA R | CCGAAGTTACGGATCCATTT |