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. 2013 Feb 26;4:1508. doi: 10.1038/ncomms2489

Figure 2. Transient overexpression of PIB5PA inhibits cell proliferation and survival.

Figure 2

(a) Whole-cell lysates from ME1007 and Mel-FH cells, either non-transfected or transfected with empty vector-HA or PIB5PA-HA constructs, were subjected to western blot analysis. Data shown are representative of three individual experiments. (b–e) ME1007 and Mel-FH cells were transiently transfected with empty HA, PIB5PA-HA, or mutant PIB5PA (PIB5PASKICH)-HA constructs. (b) Seventy-two hours later, apoptotic cells were quantitated by measurement of sub-G1 DNA content (n=3, mean±s.e.m.). (c) Forty-eight hours later, whole-cell lysates were subjected to western blot analysis. Data shown are representative of three individual experiments. (d) Seventy-two hours later, cells were subjected to proliferation assays using the BrdU incorporation method (n=3, mean±s.e.m.). (e) Twenty-four hours later, viable cells (2,000 cells per well in six-well plates) were allowed to grow for 12 days before being fixed with methanol and stained with crystal violet. Data shown are representative of three individual experiments. Scale bar, 1 cm.