Figure 6. Other mutations at the intrasubunit interfaces between different domains.

(a) C36R (Protein Data Bank (PDB) ID: 4I0Y). (b) V219I (PDB ID: 4I8M). (c) I404M (PDB ID: 4I2S). Colour scheme as in Fig. 4. For I404M, dual conformations were observed in the electron density. Arrows indicate changes relative to the wild-type structure. (d) RMSD values for superposition of the mutants with the wild-type structure. The superposition is performed based on domain A, so the values for domains B and C are the sum of intrinsic structural differences within the domains and relative domain reorientations compared with the wild-type structure. R402G and R45C are in comparison with the wild-type structure crystallized in PA5100.