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. 2013 Feb 26;4:1518. doi: 10.1038/ncomms2504

Figure 6. Sequence alignment of C3 and C4 determining regions in various PEPCs.

Figure 6

Amino acids that determine C3/C4-specific function are highlighted. In the substrate-binding centre, Ala774 (Flaveria numbering) mediates C3 specificity, while Ser774 determines the increased kinetic efficiency of C4 PEPC (black boxes). Malate binding in the inhibitory site is controlled by Arg884 in the C3 enzyme, while the increased tolerance of the C4 PEPC towards the feedback inhibitor is mediated by Gly884 (grey boxes). The sequences are: Ft (F. trinervia UniProt No. P30694), So (Saccharum officinarum Q9FS96), Sb (Sorghum bicolor P15804), Zm (Zea mays P04711), Fp (F. pringlei GenBank CAA88829.1), Os (Oryza sativa subsp. indica Q84XH0), Nt (Nicotiana tabacum P27154) and At (Arabidopsis thaliana Q84VW9).