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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: Free Radic Biol Med. 2010 Jan 22;48(8):1051–1063. doi: 10.1016/j.freeradbiomed.2010.01.021

Fig 2.

Fig 2

Effect of SFN on NRF2 activity in NRK cells. (A) Transcript levels of NRF2-target genes (NQO1, GCLC, and GCLM) and HPRT were measured using real-time PCR analysis following treatment of NRK cells with vehicle (Veh) or 1.25 μM SFN for 24 h. Level of HO-1 transcripts was assessed 6 h after treatment with SFN. Fold inductions are ratios over vehicle control following normalization by HPRT levels. Values are means ± S.E. from three experiments. aP<0.05 compared with vehicle-treated cells. (B) Nuclear levels of NRF2 protein and lamin B were determined by immunoblot analysis following treatment with vehicle (Veh) or SFN (0.63 or 1.25 μM) for 6 h. (C) ARE-driven luciferase activities following treatment with CsA. NRK cells were transfected with the luciferase reporter plasmid containing the NQO1 ARE and were treated with vehicle (Veh) or SFN (0.63 or 1.25 μM) for 24 h. Measurement of luciferase activities was performed by the Dual Luciferase System using the Renilla luciferase control plasmid. Values are means ± S.E. from four experiments. aP<0.05 compared with vehicle-treated control cells.