Figure 5.

TRPC6 channels are often localized intracellularly in the axons of dentate granule cells. A1-2: Gold particles representing TRPC6 channels were mainly found in the en passant axons, frequently forming bundles in the stratum lucidum (arrowheads). In the mossy terminals, gold labeling avoiding vesicular clusters was commonly attached to the plasma membrane or intracellular membrane cisternae (arrows). Inserts show the same structure taken from the bottom left parts of the consecutive sections, highlighting a gold particle attached to an intracellular membrane cisterna. B, C, D: High-power electron micrographs demonstrating immunogold staining associated with membrane cisternae (open arrows). Scale bars: A, 0.5 µm; insert 0.25 µm; B-D 0.2 µm.