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. 2013 Feb 5;2(2):e70. doi: 10.1038/mtna.2012.61

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Copyright © 2013 American Society of Gene & Cell Therapy

Molecular Therapy-Nucleic Acids is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Plasmid design and protein production. (a) Vector constructs of the non-optimized (top) and optimized (bottom) CBhEqIL-1ra-pTRs-ks plasmids (left) and the CMVEqIL-1ra-pTRs-ks plasmids (right). Specific base pair sequence changes were made in the optimized IL-1ra gene (without affecting the protein sequence) so that the protein would be produced more efficiently by the scAAV construct. (b) IL-1ra production in transduced 293 cells. IL-1ra production was assessed in 293 cells transduced with 2 µg/cm of optimized and non-optimized equine IL-1ra plasmid constructs. CMV, cytomegalovirus; IL, interleukin; scAAV, self-complementary adeno-associated virus.