Figure 3.

Allogeneic immune responses elicited by wild-type and transduced cells. CSFE-labeled peripheral blood mononuclear cells (PBMCs) were incubated with medium alone, or cocultured with control allogeneic PBMCs, wild-type or transduced cells. (a) Activation of CD3⁺CD4⁻ T cells as determined by the expression of CD25. (b) Proliferation of CD3⁺CD4⁻ T cells as evaluated by determining the percentages of dividing T cells. Results are expressed as mean values ± SD for three independent experiments each performed in triplicate at different times of culture (*P < 0.05). Note: Control allogeneic PBMCs triggered large responses both in terms of activation and proliferation, as expected (positive control).