Table 2.
Relaxant effect of extract, essential oil and fractions from Rosa damascena in comparison with negative control (saline) and positive control (theophylline) in group 1 experiments (KCl) (66, 67, 70).
| Different Solution | Concentration | G1 | G2 | G3 |
|---|---|---|---|---|
| 0.25 | 5.60±2.42 | 1.62±1.16 | 0.00±0.00 | |
| Ethanolic extract | 0.50 | 11.60±4.95 | 17.12±4.06 | 0.00±0.00 |
| 0.75 | 20.00±9.12 | 43.25±6.32 | 4.00±3.00 | |
| 1.0 | 41.60±11.95 | 60.37±6.98 | 9.00±5.00 | |
| 0.25 | 22.80±6.38 | 15.19±1.57 | 0.00±0.00 | |
| Essential oil | 0.50 | 32.40±7.36 | 38.50±4.25 | 2.00±2.00 |
| 0.75 | 53.80±7.91 | 59.13±7.47 | 0.00±0.00 | |
| 1.0 | 82.40±7.92 | 67.88±6.27 | 8.00±5.00 | |
| 0.1 | -3.50±1.17 | 18.25±2.40 | - | |
| Aqueous F. | 0.2 | -6.30±0.50 | 26.75. ±3.32 | - |
| 0.4 | -6.60±0.98 | 34.88±4.37 | - | |
| 0.1 | 33.80±2.13 | 21.50±5.37 | - | |
| Ethyl acetate F. | 0.2 | 48.20±3.50 | 44.81±11.55 | - |
| 0.4 | 68.42±4.48 | 77.89±9.14 | - | |
| 0.1 | 3.48±1.20 | 1.56±0.87 | - | |
| N-buthanol F. | 0.2 | 6.20±0.46 | 3.50±0.87 | - |
| 0.4 | 24.00±3.77 | 5.00±1.17 | - | |
| 0.25 | -4.36±2.44 | -1.92±0.27 | - | |
| Theophylline | 0.50 | 17.81±7.44 | 12.43±1.63 | - |
| 0.75 | 50.40±6.86 | 33.26±3.02 | - | |
| 1.0 | 88.20±7.28 | 73.81±4.53 | - |
Values are presented as mean±SEM. The unit of concentration for essential oil was vol%, for extract and fractions was g%, and for theophylline was mM. Group 1 (G1); KCl induced contraction on non - incubated tracheal chains (n= 5), Group 2 (G2); methacholine induced contraction on non - incubated tracheal chains (n= 8) and Group 3 (G3); methacholine induced contraction on incubated tracheal chains of guinea pig with propranolol and chlorpheniramine (n= 5).