FIGURE 3.

SnL upregulation on CD4⁺ T cells is associated with increased α2,3 sialylation. (A) Splenocytes from C57BL/6 mice (n = 4) were activated for 72 h with plate-bound anti-CD3 (5 μg/ml). Activated cells (solid lines) were compared with freshly isolated splenocytes (dashed lines) for biotinylated MAL and biotinylated SNA binding. Streptavidin only was used as a control for MAL and SNA (solid gray histograms). (B) Mean fluorescence intensities (MFI) of Sn–Fc (top panel), MAL (middle panel), and SNA (bottom panel). CD4⁺Foxp3⁻ T cells are depicted as dark bars and MFIs of fresh and 72-h postactivation CD4⁺Foxp3⁻ T cells are depicted as light bars. Data represent means ± SEM from four mice per activation status. Data are representative of two independent experiments. Statistical analysis was done using one-way ANOVA with Bonferroni posttest. *p < 0.05, **p < 0.001.