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. 2013 Feb 13;190(6):2593–2602. doi: 10.4049/jimmunol.1201172

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Copyright © 2013 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 4. — Sn–Fc binds SnL on activated CD4⁺ T cells in α2,3-specific manner. (A) Activated CD4⁺ T cells were treated with α2,3-specifc sialidase L (dashed gray histograms), nonspecific sialidase (solid gray histogram), or left untreated (black histogram). (B) Similar treatments to (A) but with surface labeling for SNA. (C) Proteinase K (gray unfilled histogram) compared with buffer-only treatment (black histogram) abolishes Sn-Fc binding to activated CD4⁺ T cells. Data are representative of two independent experiments.