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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Br J Dermatol. 2013 Jan 31;168(3):595–601. doi: 10.1111/bjd.12096

Fig. 3.

Fig. 3

The influence of different UV wavebands radiation on cortisol activity−regulating enzymes and GR expressions is shown by double IF performed on skin explants (a) and co-cultured HEKn/HEMn (b).

Tissues and cells were collected 24 h after exposure. The intensity of green (FITC; 11β-HSD1 or 11β-HSD2) or red (CY3; GR) signals is proportional to the expression levels of analyzed antigens and showed on inserted graphs to Fig. 3b. The statistical (one-way Anova Dunnett’s test) differences between conditions were marked with red (for GR) and green (for 11β-HSD1 or 11β-HSD2) asterisks on inserted graphs. Double headed arrows indicate the nuclear localization of GR while single headed arrows show cytoplasmic localization of 11β-HSD1/D2 antigens.