Table 2.
Specification of antibody and reagents used for WB and dIF.
| Primary antibody | Host | Vendor |
|---|---|---|
| Hydroxysteroid dehydrogenase type 1, 11β-HSD1 | sheep | Binding Site, Birmingham, UK |
| Hydroxysteroid dehydrogenase type 2, 11β-HSD2 | sheep | |
| Glucocorticoid receptor, GR | rabbit | Santa Cruz Biotech., CA |
| Secondary antibody and reagents | ||
| Biotinylated anti rabbit IgG | donkey | Jackson ImmunoRes., West Grove, PA |
| Anti sheep IgG−FITC | donkey | |
| Streptavidin−CY3 | − | |
| Anti sheep IgG−HRP | donkey | Santa Cruz Biotech., CA |
| Anti rabbit IgG−HRP | donkey | |
| β-actin IgG−HRP | mouse | Sigma, St. Louis, MO |