Table 2.
Primary antibody | Host | Vendor |
---|---|---|
Hydroxysteroid dehydrogenase type 1, 11β-HSD1 | sheep | Binding Site, Birmingham, UK |
Hydroxysteroid dehydrogenase type 2, 11β-HSD2 | sheep | |
Glucocorticoid receptor, GR | rabbit | Santa Cruz Biotech., CA |
Secondary antibody and reagents | ||
Biotinylated anti rabbit IgG | donkey | Jackson ImmunoRes., West Grove, PA |
Anti sheep IgG−FITC | donkey | |
Streptavidin−CY3 | − | |
Anti sheep IgG−HRP | donkey | Santa Cruz Biotech., CA |
Anti rabbit IgG−HRP | donkey | |
β-actin IgG−HRP | mouse | Sigma, St. Louis, MO |