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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: J Mol Med (Berl). 2012 Oct 23;91(4):497–506. doi: 10.1007/s00109-012-0965-1

Figure 6. Growth of tumor from tumor spheroids in dorsal skin fold window chamber implants and galectin-1 expression on radiation exposure.

Figure 6

(A) (i) Phase contrast and (ii) Fluorescence images of 7 day GFP-4T1-2H11 tumor-endothelial spheroid grown in a hanging drop of medium. (B) Elevated galectin-1 expression in 4T1-2H11 tumor-endothelial cell spheroids when compared to 4T1 tumor cell spheroids alone which is further induced in response to radiation exposure. CD34 is the tumor endothelial cell marker and Actin serves as the loading control. (C) Galectin-1 expression in lysates from tumors originating from spheroids in dorsal skin fold window chamber implants of nude mice. (D) Induction of galectin-1 expression in sections from tumors originating from tumor-endothelial cell spheroids in rear limb of nude mice upon radiation exposure of 2 Gy.