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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Mol Cell Neurosci. 2012 Nov 10;52:106–116. doi: 10.1016/j.mcn.2012.11.003

Figure 2. EphB2 is required for ephrinB2-induced cortical neuron growth cone collapse.

Figure 2

(A) Growth cone collapse assay from EphB1−/−, EphB2−/−, EphB3−/−, EphB2T-lacZ/T-lacZ, and wild-type cultured mouse cortical neurons. Cultured neurons (E16.5 + 3 DIV) were treated with 1 μ/ml clustered Fc or ephrinB2/Fc for 1 hour prior to fixation, staining and blinded scoring (***p<0.001, Two-way ANOVA).

(B) Expression of EphB2 rescues the EphB2 deficit in ephrinB2/Fc induced GCC. EphB2−/− cortical neurons were transfected with pcDNA3-Flag-EphB2 or vector alone following dissociation. After ~72 hours in culture, the neurons were treated with clustered ephrinB2/Fc for 1 hr prior to fixation and analysis of GCC as in 3A (***p<0.001, Two-way ANOVA).