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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: J Immunol. 2009 Nov 1;183(9):5575–5581. doi: 10.4049/jimmunol.0901988

FIGURE 1.

FIGURE 1

FAIM expression is regulated by IRF consensus sites. A, The locations of three IRF binding sites and one SP1 binding site within sequence 2 kb upstream of the FAIM start site are shown. B, A20 cells were transiently transfected with an empty firefly luciferase construct, or with either native, truncated, or mutant Faim promoter-firefly luciferase reporter constructs, as indicated, along with, in each case, a thymidine kinase promoter-dependent Renilla luciferase expression vector, after which lysates were prepared, firefly luciferase activity was measured in relation to the Renilla luciferase control, and results were reported as multiples of the activity present with empty vector. Mean values for three independent experiments are shown, along with lines corresponding to the SEM. C, A20 cells were transiently transfected with an empty firefly luciferase construct, or with native and mutant Faim promoter-firefly luciferase constructs with or without a CMV-driven IRF4 expression vector, as indicated, along with, in each case, a thymidine kinase promoter-dependent Renilla luciferase expression vector, after which lysates were prepared, firefly luciferase activity was measured in relation to the Renilla luciferase control, and results were reported as multiples of the activity present with empty vector. Mean values for three independent experiments are shown, along with lines corresponding to the SEM.