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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: J Immunol. 2009 Nov 1;183(9):5575–5581. doi: 10.4049/jimmunol.0901988

FIGURE 2.

FIGURE 2

Stimulated FAIM expression is preceded by induction of IRF4 expression in primary B cells. A, Primary B cells were stimulated by CD40L for 48 h alone (NS), or were stimulated by CD40L for 48 h during which time either anti-Ig, LPS, or IL-4 was added for the final number of hours indicated. From these cells, RNA was prepared, reverse transcribed, and evaluated for Faim and for Irf4 gene expression by real-time PCR. Expression levels were normalized to β2-microglobulin. Mean values for two independent experiments are shown, along with lines corresponding to the SEM. B, Primary B cells were stimulated by CD40L for 48 h alone (0 h), or were stimulated by CD40L for 48 h during which time anti-Ig was added for the final number of hours indicated. From these cells, lysates were prepared and evaluated for expression of FAIM-S and IRF4 by Western blotting. Blots were stripped and reprobed for tubulin content as a loading control. One of two comparable experiments is shown.