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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: J Immunol. 2009 Nov 1;183(9):5575–5581. doi: 10.4049/jimmunol.0901988

FIGURE 3.

FIGURE 3

IRF4 binds the Faim promoter in stimulated primary B cells. Primary B cells were stimulated by CD40L for 48 h alone (0 h) or were stimulated by CD40L for 48 h during which time anti-Ig was added for the final number of hours indicated and ChIP assay was carried out. Cells were lysed and DNA was sheared by sonication, after which chromatin was immunoprecipitated with anti-IRF4 Ab or control IgG, and Faim promoter sequence was amplified by real-time PCR. Results are expressed as the degree of amplification relative to input DNA. Mean values for two independent experiments are shown, along with lines corresponding to the SEM.