Table I.
Oligonucleotide sequences used in this studya
| Primer | Sequence |
|---|---|
| Quantitative PCR | |
| β2-microglobulin (forward) | CTGACCGGCCTGTATGCTAT |
| β2microglobulin (reverse) | TTTTCCCGTTCTTCAGCATT |
| FAIM (forward) | TGCAATGGTCAGAAAATGGA |
| FAIM (reverse) | CGCTGCTCACAGCTTTTATG |
| IRF4 (forward) | CTACCCCATGACAGCACCTT |
| IRF4 (reverse) | CCAAACGTCACAGGACATTG |
| AID (forward) | CAGGAAGCTGAGGCAGGAGG |
| AID (reverse) | TCATTTCCTTGCCACGGTCTT |
| ChIP assay: Sequence | |
| λ3′E promoter (forward) | CTTGAGAGTCCACAAGCTAAA |
| λ3′E promoter (reverse) | CTGCTAATGGACTTGGTTTC |
| FAIM promoter (forward) | AAAATCTCTCATTGGTTCGTTCC |
| FAIM promoter (reverse) | ACAAACACACAACCACTGAATTG |
| Cloning into pGL | |
| FAIM promoter −598-KpnI (forward) | CGGGGTACCCCGCACGACCTCACCCAGGATAACTAAACGCCC |
| FAIM promoter −1631-KpnI (forward) | CGGGGTACCCCGCATTTGCACAAAACCTATATGCCCAGCCCC |
| FAIM promoter XhoI (reverse) | ATACCGCTCGAGCGGTCAACGAGACCCCGCCCACTGCCCTA |
| Introduction of mutation | |
| FAIM promoter mutation A (forward) | GCATTTTAAAAATCTATTCATAGAGGCTCTTTTATGTAAGGGGTAC |
| FAIM promoter mutation A (reverse) | GTACCCCTTACATAAAAGAGCCTCTATGAATAGATTTTTAAAATGC |
| FAIM promoter mutation B (forward) | ATGTTGCAGATACCGTGCCTGAGGCTTCTGCTTCATTAAATTTTAC |
| FAIM promoter mutation B (reverse) | GTAAAATTTAATGAAGCAGAAGCCTCAGGCACGGTATCTGCAACAT |
| FAIM promoter mutation C (forward) | TTCCCTTTATATAAATGAGGCTGTAGATTCTATCCGAC |
| FAIM promoter mutation C (reverse) | GTCGGATAGAATCTACAGCCTCATTTATATAAAGGGAA |
a
All sequences are presented in the 5′ to 3′ direction.