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. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Biochemistry. 2013 Feb 6;52(7):1249–1259. doi: 10.1021/bi301500d

Figure 4.

Figure 4

SDS-PAGE of the fiber protein extract and purified proteins. Lane 1 is the untreated fiber control, lane 2 the E134A-PAGFP exchanged fiber, and lane 3 purified proteins identified on the right of the gel except for ELC and porcine RLC. Quantitative evaluation of the untreated and exchanged fibers was done with ImageJ and using the ELC content as standard. Error is ~10%. Stoichiometric replacement of the porcine RLC is indicated by the sum of Lane 2 bands at HCRLC-PAGFP and porcine RLC equaling 1 to within error. HC is heavy chain and TnC is troponin C.