Skip to main content
. Author manuscript; available in PMC: 2014 Feb 19.
Published in final edited form as: Biochemistry. 2013 Feb 6;52(7):1249–1259. doi: 10.1021/bi301500d

Table 2.

Sub-state occupation probability, maximum isometric tension, and cross-bridge stiffness for HCRLC-PAGFP exchanged permeabilized papillary muscle fibers. 1

State WT M20L E134A G162R
A- 0.054 ± 0.003 0.22 ± 0.04 0.128 ± 0.002 0.08 ± 0.03
B- 0.25 ± 0.07 0.35 ± 0.10 0.28 ± 0.01 0.44 ± 0.07
C- 0.26 ± 0.05 0.28 ± 0.02 0.31 ± 0.02 0.24 ± 0.02
D- 0.18 ± 0.02 0.11 ± 0.06 0.20 ± 0.04 0.18 ± 0.06
E- 0.25 ± 0.01 0.05 ± 0.02 0.08 ± 0.04 0.06 ± 0.01
Fat 1. 1.05 ± 0.07 0.85± 0.07 1.00 ± 0.06
Relative stiffness 0.44 ± 0.02 0.32 ± 0.02 0.33 ± 0.01 0.35 ± 0.01
1

Errors represent standard error of the mean for n = 3-4. The full data set for single molecule dipole orientations was partitioned into n = 3 or 4 parts then occupation probabilities computed for each part. The n results were averaged and the standard error of the mean computed. Fat is peak isometric tension normalized to the WT fiber value. Relative stiffness is computed using eq. 4 and Fat.