Table 2.
State | WT | M20L | E134A | G162R |
---|---|---|---|---|
A- | 0.054 ± 0.003 | 0.22 ± 0.04 | 0.128 ± 0.002 | 0.08 ± 0.03 |
B- | 0.25 ± 0.07 | 0.35 ± 0.10 | 0.28 ± 0.01 | 0.44 ± 0.07 |
C- | 0.26 ± 0.05 | 0.28 ± 0.02 | 0.31 ± 0.02 | 0.24 ± 0.02 |
D- | 0.18 ± 0.02 | 0.11 ± 0.06 | 0.20 ± 0.04 | 0.18 ± 0.06 |
E- | 0.25 ± 0.01 | 0.05 ± 0.02 | 0.08 ± 0.04 | 0.06 ± 0.01 |
Fat | 1. | 1.05 ± 0.07 | 0.85± 0.07 | 1.00 ± 0.06 |
Relative stiffness | 0.44 ± 0.02 | 0.32 ± 0.02 | 0.33 ± 0.01 | 0.35 ± 0.01 |
Errors represent standard error of the mean for n = 3-4. The full data set for single molecule dipole orientations was partitioned into n = 3 or 4 parts then occupation probabilities computed for each part. The n results were averaged and the standard error of the mean computed. Fat is peak isometric tension normalized to the WT fiber value. Relative stiffness is computed using eq. 4 and Fat.