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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Brain Behav Immun. 2012 Dec 20;29:70–81. doi: 10.1016/j.bbi.2012.12.004

Fig. 5.

Fig. 5

Muc1 deficiency in CD4+ T cells does not promote Th1 and Th17 differentiation.

CD4+ T cells (2×106 cells/ml) were purified from the spleens of wt and Muc1−/− mice. (A) CD4+ T cells were cultured for 72 h in the presence of plate-bound anti-CD3 antibody (5 μg/ml), soluble anti-CD28 antibody (1 μg/ml) plus IL-12 for Th1 differentiation, or with IL-6, TGF-β, and anti-IL-4 and anti-IFNγ antibodies for Th17 differentiation. Supernatants were collected and IFNγ (A) and IL-17 (B) production was determined by ELISA. Data represent mean ± SE (n = 4). One representative experiment of three is shown.