Skip to main content
. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Brain Behav Immun. 2012 Dec 20;29:70–81. doi: 10.1016/j.bbi.2012.12.004

Fig. 6.

Fig. 6

Muc1 deficiency in splenic DC augments Th1 and Th17 differentiation.

CD11c+ cells were purified from the spleens of wt and Muc1−/− mice, stimulated with LPS (1 μg/ml) for 14 h and pulsed with MOG (50 μg/ml; specific antigen) or PLP (50 μg/ml; nonspecific antigen-control) for 3 h. After extensive washing, CD11c+ cells were cocultured with MOG-specific naïve CD4+ T cells purified from the spleens of 2D2 mice (2×106cells/ml, DC:T cell=1:10). After 72 h, cells were subjected to FACS for intracellular IFNγ and IL-17 staining (A). Supernatants were collected for IL-17 ELISA after 72 h and 96 h coculture (B). Proliferation of CFSE-labeled MOG-specific CD4+ T cells was measured at 72 h or 96 h (C). Splenocytes from wt or Muc1−/− mice were harvested and treated with LPS for 24 h. Cells were subjected to surface staining for CD11c, CD40, CD80, CD86 and MHCII. One representative experiment of three (A-C) or of two (D) is shown.