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. Author manuscript; available in PMC: 2013 Mar 4.
Published in final edited form as: Anal Bioanal Chem. 2008 Apr 24;391(5):1917–1930. doi: 10.1007/s00216-008-2095-8

Fig. 3a–b.

Fig. 3a–b

CID spectra of 1α,25(OH)2D3. Native 1α,25(OH)2D3 a; 1α,25(OH)2D3-PTAD b. Both product ion spectra were acquired for the dominant [M–18+H]+ ion. The general fragmentation reaction for derivatized vitamin D metabolites is shown. We observed a dominant fragment for PTAD derivatives at m/z 298 (m/z 314 for metabolites hydroxylated at position 1)