Skip to main content
. 2013 Jan 22;110(9):3333–3338. doi: 10.1073/pnas.1214266110

Fig. 5.

Fig. 5.

SIRT1 activators repress circadian gene expression in a SIRT1-specific manner. (A) Per2, Dbp, and Cry1 mRNA expression profiles in WT MEFs pretreated with DMSO, SRT CD1023, CL1015, and CE1022 (10 μM), before serum shock synchronization, were analyzed by quantitative PCR. The values are relative to those of Gapdh mRNA levels at each circadian time. Time 0 value in DMSO-treated cells was set to 1. All of the values are the mean ± SEM (n = 3); *P < 0.05, **P < 0.01. (B) Cross-linked cell extract were isolated at the indicated time points after serum shock from WT MEFs pretreated with SRTCD1023, CL1015, and CE1022 (10 μM). The samples were subjected to ChIP assay with anti-CLOCK and anti-IgG (ctr), and analyzed by quantitative PCR with primers for Per2 and Dbp promoter. All of the values are the mean ± SD (n = 3); *P < 0.05, **P < 0.01, ***P < 0.001. (C) Same experimental conditions as in B, but using SIRT1−/− MEFs.