Fig. 5.

GA Inhibition of vacuolar PIN trafficking downstream of BFA action. The PIN2::PIN2-GFP signal in BFA endosomes was imaged after treatment with DMSO for 3 h (GA control) (A), 50 µM GA for 3 h (B), DMSO for 2 d (uniconazole control) (D), or 10 µM uniconazole for 2 d (E). (G and H) PIN2-GFP signal in BFA endosomes of (G) PIN2::PIN2-GFP and (H) PIN2::PIN2-GFP × ga1-3 seedlings. (C, F, and I) Quantification of PIN2-GFP signal at the PM relative to endosomal signals in seedlings treated as described in A, B, D, E, G, and H. Data represent means ± SD (n = 7 roots were analyzed per treatment, and, in six cells per root, endosomal signal was quantified; *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ 0.001, treatment vs. control). (Scale bar: 50 μm.)