Skip to main content
. 2013 Feb 1;110(9):3483–3488. doi: 10.1073/pnas.1222435110

Fig. 3.

Fig. 3.

HIF-2α is sufficient to activate CDCP1 and promote tumor growth in xenografts. (A) Tumor formation over time in nude mice injected with the A375 cancer cell line expressing GFP or HIF-2αDPA. Doxycycline chow was used to induce expression of GFP or HIF-2αDPA. Error bars are SEM. (n = 6). (B) Representative images of tumors; GFP control cells (left flank) or HIF-2αDPA (right flank) after mice were euthanized. (C) Protein was isolated from tumors and immunoblotted for CDCP1, P-734 CDCP1, HA, and α-tubulin for loading control. Shown are two representative tumors from the mice. (D) Scatterplot shows that overexpression of HIF-2α significantly enhanced lung metastases in NOD/SCID mice. The number of mice with surface lung metastases was counted 9 wk after tail-vein injections (n = 4). (E) Heat map of CDCP1 and HIF-2α expression based on the microarray data of 732 unique cancer cell lines from the SCLP. (F) Scatterplots comparing the expression levels of CDCP1 with HIF-2α (Left), EGFR (Center), and MET (Right), all showing a strong positive correlation in their expression across the 732 cancer cell lines (P = 1 × 10−20, Pearson’s correlation coefficient analysis). (G) Heat map of CDCP1, HIF-2α, EGFR, and MET expression across the SCLP (n = 732).